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Research Projects/Laser Treatment
Laser treatment can significantly reduce cholesterol, triglycerides, study finds
A new study, designed to evaluate the efficacy of laser therapy for reducing cholesterol and triglyceride serum levels, revealed that 75 percent of participants realized an overall reduction in cholesterol serum of 16.1 points. Ryan Maloney, medical director of Erchonia Medical Inc. (McKinney, TX) is reporting the research results at the Annual Conference of the American Society for Laser Medicine and Surgery (ASLMS), which opens today in National Harbor, MD.
Maloney says his clinical study was originally designed to investigate how dissolved fat affects body chemistry. But while monitoring lipid and cholesterol levels, the research team discovered a trend indicating that laser light could disrupt cholesterol formation. "We know that cholesterol and its production are highly regulated by transcription factors, so the possibility to alter cholesterol serum levels theoretically exists," said Maloney. "Since low-level laser therapy was proven to affect transcription factors, we wanted to determine if laser therapy could serve as a subtle, non-invasive instrument to lower cholesterol and triglyceride serum levels."
Twenty volunteers between the ages of 18 and 65 participated in the non-controlled, non-randomized study. Participants received low-level laser treatments three times per week for two weeks, with each treatment session lasting approximately 40 minutes. Treatments were administered across the abdomen and waist area, and wrapping around the lower back--an area that generally contains the most concentrated pockets of subcutaneous fat. The laser produced 17.5 milliwatts of energy at 635 nanometers.
The 75 percent of study participants that demonstrated overall reduction in cholesterol serum levels, had a reduction ranging from -1.0 to -31.0 mg/dL. For those participants demonstrating an overall reduction in cholesterol serum levels, 93 percent experienced a reduction in LDL levels (commonly referred to as "bad cholesterol"), with 47 percent revealing a reduction in LDL levels without experiencing a reduction in HDL levels (or "good cholesterol"). Of the 20 participants, 60 percent demonstrated a reduction in triglyceride levels.
"We were incredibly surprised by these findings, especially given that we stumbled upon this observation by accident," said Maloney. "It's exciting to see laser technology shift in the direction of treating chronic conditions with the potential to one day serve as a viable alternative to leading prescription medications used to treat high cholesterol."
Maloney said this research is now moving into a randomized placebo-controlled, double-blinded clinical trial expected to begin at multiple sites later this year. The clinical trial will investigate, among other things, the long-term effects of low-level laser irradiation on cholesterol and triglyceride serum levels in hopes of establishing treatment guidelines to ensure levels are maintained over time. "Low-level laser therapy is gaining popularity across the medical community as an effective form of preventative medicine, and I think this trend will certainly continue in the future," he said.
 Laser Blood Irradiation Therapy/Transdermal Systemic Laser Therapy (TDSLT)
…heal and increase functioning efficacy of the vascular, immune and respiratory system:
Currently the methods of laser and non-laser (incoherent monochromic, narrow-band or broadband) light blood irradiation therapy - the methods of photo-hemotherapy - are widely applied in the treatment of different pathologies. Direct intravenous and extracorporeal (with red, UV and blue light) as well as non-invasive transcutaneous (with red and infrared light) irradiation of blood are used. Unlike the treatment mechanisms of local laser therapy, the medical effects of photo-hemotherapy methods are determined by predominance of systemic healing mechanisms above the local ones, increasing the functioning efficacy of vascular, respiratory, immune, other systems and organism as a whole.
… normalisation of parameters of hormonal, immune, reproductive systems
The method of laser blood irradiation was developed in 1981 by the scientists E.N. Meshalkin and V.S. Sergievskiy. Originally the method was applied in the treatment of cardiovascular pathologies. Some authors reported that the treatment possibilities of the method are very large and include the improvement of rheological characteristics of the blood and microcirculation, normalisation of parameters of hormonal, immune, reproductive and many other systems.
… antitoxic, bio-stimulative, immuno-corrective, anti-allergic, antibacterial, antiviral, analgetic, anti-inflammatory
The laser stimulates the immune response of the organism, activates erythrogenesis and improves deformability of erythrocyte membranes, has anti-hypoxic activity on tissues and general antitoxic influence on the organism at different pathological processes. Our laser is used for its bio-stimulative, analgetic, antiallergic, immunocorrective, antitoxic, vasodilative, antiarrhythmic, antibacterial, antihypoxic, spasmolytic, anti-inflammatory and some other properties.
…boost the cellular part of your immunity, decrease concentrations of microbes in the abdomen, activate microcirculation
The laser activates non-specific mechanisms of anti-infectious immunity. Intensifying of bactericidal activity of serum of the blood and system of the complement, reduction of the degree of C - reactive protein, level of average molecules and toxicity of plasma, increasing the content of IgA, IgM and IgG in the serum of the blood, as well as decreasing of the level of circulating immune complexes are proved. There are studies on boosting effects of the laser on the cellular part of immunity (N. F. Gamaleya et al., 1991). Under influence of the laser, the phagocytic activity of macrophages markedly increases, concentration of microbes in the abdomen of patients decreases, reduction of inflammatory exhibiting of disease, activation of microcirculation are detected. The medical effect of the laser is stipulated by its immuno-corrective activity by normalisation of intercellular relationships within the subpopulation of T-lymphocytes and increasing the amount of immune cells in a blood. It elevates the function activity of B-lymphocytes, strengthens the immune response, reduces the degree of intoxication and as a result improves the general condition of patients (V. S. Sergievskiy et al., 1991).
…increase energy and normalise tissue metabolism, activate ATP–synthesis and energy formation in cells, increase oxidation of energy-carrying molecules
The laser promotes improving the rheological properties of blood, rising fluidity and activating transport functions. That is accompanied by increasing the oxygen level, as well as decreasing the carbon dioxide partial pressure. The arterio-venous difference by oxygen is enlarged, that testifies the liquidation of a tissue hypoxia and enrichment the oxygenation. It is a sign of normalisation of tissue metabolism. Probably, the basis of activation of oxygen transport function of the laser is the influence on haemoglobin with transforming it in more favourable conformation state. The augmentation of oxygen level improves metabolism of the organism tissues. In addition, the laser irradiation activates the ATP synthesis and energy formation in cells (A. S. Krjuk et al., 1986). Application of IV LBI in cardiology has shown that procedures have analgetic effect, show reliable rising tolerance of patients towards physical tolerance test, elongation of the period of remission. It was proved that the laser reduces aggregation ability of thrombocytes, activates fibrinolysis, which results in peripheral blood flow velocity increasing and tissues oxygenation enriching. The improvement of microcirculation and utilisation of oxygen in tissues as a result of the laser is intimately linked with positive influence on metabolism: higher level of oxidation of energy-carrying molecules of glucose, pyruvate, and other substances.
… unblocking of capillaries, positive pre- and post-surgical operations effects
The improvement in microcirculation system is also stipulated by vasodilation and change in rheological properties of blood as a result of drop of its viscosity, decrease of aggregation activity of erythrocytes due to changes of their physicochemical properties, in particular rising of negative electric charge. Finally the activation of microcirculation, unblocking of capillaries and collaterals, improvement of tissue trophical activity, normalisation of a nervous excitability take place (N. N. Kapshidze et al., 1993). TSLT is recommended to apply before surgical operations as preparation for intervention, as well as in the postoperative stage, because the laser irradiation of blood has not only analgetic effect, but also spasmolytic and sedative activity.
…regeneration and reparation of cells, incl. inner genital organs
Our laser procedures have found broad application in obstetrics and gynaecology for activation the blood flow in utero-placental and feto-placental basins, for prophylaxis of the pathologies at delivery, for influence on inflammatory processes of inner genital organs. The laser normalises production of gonadotropins, improves microcirculation, elevates oxygen pressure in blood and in tissues, and so accelerates the process of regeneration and reparation.
…increase functional activity of the hypothalamus and positive effects on the activation of energetic, metabolism, immune and vegetative responses.
In order to explain the generalised and multifactor effects of the laser, its positive influence practically on all tissues and functional systems of the body, clinical effectiveness for the treatment of different diseases, some authors mentioned that the improvement of microcirculation after TSTL is detected in all structures of central nervous system, but this improvement is most active in the hypothalamus, which has highly developed vascular system. The capillaries of a hypothalamus are remarkable for high permeability for macro-molecular proteins, which should even more amplify influence of the irradiated blood to subthalamic nuclei. So it is supposed, that TSLT increases the functional activity of hypothalamus and all limbic system, and as a result the activation of energetic, metabolism, immune and vegetative responses, mobilization of adaptive reserves of an organism is reached.
Laser Photons and Pharmacological Treatments in Wound Healing
Farouk A.H. Al-Watban, MSc, PhD, and Bernard L. Andres, MT(AMT) Laser Medicine Research Section, Biological and Medical Research Department, King Faisal Specialist Hospital & Research Center, Riyadh, Saudi Arabia.
The exploitation of photobiology in medicine has been of great interest to mankind. There is a growing interest in the use of lasers for treatment purposes because of the photochemical alterations induced in biomolecules by light energy. In this paper we present our data on laser biostimulation, the combination of pharmacological treatments SolcoserylTM (SS) and PolygenTM (PG) with light therapy using in-vitro and in-vivo models. In-vitro experiments indicate the ability of laser photons and pharmacological agents SS or PG to augment or abate the cloning efficiency of various cell lines. In-vivo studies focused on the dosimetry of various laser wavelengths and the use of wound healing drugs and 632.8nm laser in wound healing. The application of pharmacological treatments combined with laser therapy reveals the utility of light-drug treatment combinations. Given the ever-increasing cost of medical care, the burden incurred on patients, caregivers and society, this line of research fulfills the increasing need to develop treatment methods that enhance wound healing, especially in situations involving resistance to healing.
The Biological Effects of Laser Therapy and Other Physical Modalities on Connective Tissue Repair Processes
Chukuka S. Enwemeka, P.T., Ph.D., FACSM, G. Kesava Reddy, Ph.D., Department of Physical Therapy and Rehabilitation Sciences, University of Kansas Medical Center, Kansas City, KS 66160-7601, USA
Connective tissue injuries, such as tendon rupture and ligamentous strains, are common. Unlike most soft tissues that require 7-10 days to heal, primary healing of tendons and other dense connective tissues take as much as 6 - 8 weeks during which they are inevitably protected in immobilization casts to avoid re-injury. Such long periods of immobilization impair functional rehabilitation and predispose a multitude of complications that could be minimized if healing is quickened and the duration of cast immobilization reduced. In separate studies, we tested the hypothesis that early function, ultrasound, 632.8 nm He-Ne laser, and 904 nm Ga-As laser, when used singly or in combination, promote healing of experimentally severed and repaired rabbit Achilles tendons as evidenced by biochemical, biomechanical, and morphological indices of healing. Our results demonstrate that: (1) appropriate doses of each modality, i.e., early functional activities, ultrasound, He-Ne and Ga-As laser therapy augment collagen synthesis, modulate maturation of newly synthesized collagen, and overall, enhance the biomechanical characteristics of the repaired tendons. (2) Combinations of either of the two lasers with early function and either ultrasound or electrical stimulation further promote collagen synthesis when compared to functional activities alone. However, the biomechanical effects measured in tendons receiving the multi-therapy were similar, i.e., not better than the earlier single modality trials. Although tissue repair processes in humans may differ from that of rabbits, these findings suggest that human cases of connective tissue injuries, e.g., Achilles tendon rupture, may benefit from appropriate doses of He-Ne laser, Ga-As laser, and other therapeutic modalities, when used singly or in combination. Our recent meta-analysis of the laser therapy literature further corroborate these findings.
Thermographic Study of Low Level Laser Therapy for Acute-Phase Injury
Yoshimi Asagai, M.D.1, Atsuhiro Imakiire, M.D.2, Toshio Ohshiro, M.D.3, 1. Shinano Handicapped Children’s Hospital Shimosuwa, Nagano, Japan 2. Department of Orthopedic Surgery, Tokyo Medical University Shinjuku, Tokyo, Japan 3. Japan Medical Laser Laboratory, Shinanomachi, Tokyo, Japan
Acute-phase injury is generally treated by localized cooling of the region, and rarely by the active use of low level laser therapy (LLLT) in Japan. Thermographic studies of acute-phase injury revealed that circulatory disturbances at the site of trauma occurred due to swelling and edema on the day following the injury, and that skin temperature was high at the site of the trauma and low at the periphery. Following LLLT, circulatory disturbances rapidly improved, while temperature in the high temperature zone around the site of trauma fell by 3 degrees on the average, but at the periphery the low temperature rose by 3 degrees on the average to nearly normal skin temperature. Clinically, swelling and edema improved. LLLT was also useful in treating necrosis of the skin in the wound area and in accelerating healing of surgical wounds of paralytic feet, which are prone to delayed, wound healing and also wounds due to spoke injury. LLLT is useful in treating swelling and edema in acute-phase injury and in accelerating healing of surgical wounds.
Advances in Laser Therapy for Bone Repair
A. Barber 1, JE. Luger 1, A. Karpf 1 , Kh. Salame 2 , B. Shlomi 3,G. Kogan 3, M. Nissan 4, M. Alon 5, and S. Rochkind 2,6.
1Foot & Ankle Unit, Departments of Orthopedic Surgery "B", Departments of 2Neurosurgery, 3Oral and Maxillofacial Surgery, and 5Rehabilitation, 6Division of Peripheral Nerve Reconstruction, Tel Aviv Sourasky Medical Center, Tel Aviv University; 4Ben Gurion University, Israel.
During the last decade, it was discovered that low-power laser irradiation has stimulatory effects on bone cell proliferation and gene expression. The purposes of this review are to analyze the effects of low- power laser irradiation on bone cells and bone fracture repair, to examine what has been done so far, and to explore the additional works needed in this area. The studies reviewed show how laser therapy can be used to enhance bone repair at cell and tissue levels. As noted by researchers, laser properties, the combinations of wavelength and energy dose need to be carefully chosen so as to yield bone stimulation. With better study designs, the results will be more credible, allowing for greater recognition of advances in bone repair using laser therapy. Many studies on the effects of laser therapy on bone healing and fracture repair have used biochemical and histological methods. However, in order to establish the effects of laser treatment on bone, additional studies need to be performed using biomechanical tests, the ultimate evidence of bone repair. Finally, future studies are needed to demonstrate that the same bone stimulation effects occurring in animals may also be seen in humans.
Attenuation and Penetration of Visible 632.8nm and Invisible Infrared 904nm Light In Soft Tissues
Chukuka S. Enwemeka, Ph.D., FACSM Department of Physical Therapy & Rehabilitation Sciences, University of Kansas Medical Center, Kansas City, KS, and Department of Veterans Affairs Medical Center, Kansas City, MO, U.S.A.
We studied the depth of penetration and the magnitude of attenuation of 632.8nm and 904nm light in skin, muscle, tendon, and cartilagenous tissues of live anaesthetized rabbits. Tissue specimens were dissected, prepared, and their thicknesses measured. Then, each wavelength of light was applied. Simultaneously, a power meter was used to detect and measure the amount of light transmitted through each tissue. All measurements were made in the dark to minimize interference from extraneous light sources. To determine the influence of pulse rate on beam attenuation, the 632.8nm light was used at two predetermined settings of the machine; continuous mode and 100 pulses per second (pps), at an on:off ratio of 1:1. Similarly, the 904nm infra-red light was applied using two predetermined machine settings: 292 pps and 2,336 pps. Multiple regression analysis of the data obtained showed significant positive correlations between tissue thickness and light attenuation (p < .001). Student's t-tests revealed that beam attenuation was significantly affected by wavelength. Collectively, our findings warrant the conclusions that (1) The calf muscles of the New Zealand white rabbit attenuates light in direct proportion to its thickness. In this tissue, light attenuation is not significantly affected by the overlying skin, a finding which may be applicable to other muscles. (2) The depth of penetration of a 632.8nm and 904nm light is not related to the average power of the light source. The depth of penetration is the same notwithstanding the average power of the light source. (3) Compared to the 904nm wavelength, 632.8nm light is attenuated more by muscle tissue, suggesting that is absorbed more readily than the 904nm wavelength or conversely that the 904nm wavelength penetrates more. Thus, wavelength plays a critical role in the depth of penetration of light.
Biomodulation Effects on Cell Mitosis After Laser Irradiation Using Different Wavelengths
R. Sroka, C. Fuchs, M. Schaffer, U. Schrader-Reichardt, M. Busch, T. Pongratz, R. Baumgartner LFL Laser – Research Laboratory – Clinic of Urology and Clinic of Radiotherapy, University Munic, FRG
The biostimulative effects on cell mitosis induced by laser light at different wavelengths in cell cultures was investigated. Murine skeletal fibroblasts (C2), normal urothelial cells (HCV29), human squamous carcinoma cell line of the mouth (ZMK) and urothelial carcinoma cells (J82) were irradiated with laser light at ^=488, 630, 640 and 805+25 pm using a computer controlled irradiation chamber. The irradiance was set to 10mW/cm(2) and 100mW/cm(2), while the irradiation varied between 2 and 201/cm(2). The mitotic was determined by single cell counting after Orecein staining 24h post irradiation. The mitotic rate showed a wavelength dependency with maxima at ^=635 and 805+nm for HCV29 and J82 cells. While the mitotic rate of C2 and J82 cells has the maximum value at about 41/cm(2), the maximum was at about 81/cm(2). ZMK cells showed no increase. At ^=805+25pm C2 and ZMK cells showed slight decrease in the mitotic rate after irradiation with 201/cm(2). An irradiation of 10mW/cm(2) was more effective than with 100m/Wcm(2). The biostimulation of the mitotic rate of both normal and tumor cells depends on the wavelength, irradiation and irradiance on the cell line. The wavelength dependency in the ^=630 to 640nm range could indicate a participation of endogenous porphyrins. Because the results show stimulative as well as inhibiting effects it should be considered to change the term biostimulation into “biomodulation.” Information Application: Supports laser induced biomodulation
Stimulation and Inhibition Effect of Lasers for Wound Healing on Rats
Farouk AH Al-Watban, Msc PHD and Xing Y Zhang, M.D. Laser Research, KFSH&RC, Kingdom of Saudi Arabia
The comparison of wound healing stimulation effects on rats using HeCd, Argon, He Ne, and GaAIAs lasers (for 0.39 cm 2 wound size and three times per week treatment schedule) were carried out. The inhibition effect of low power Argon laser of wound healing was also investigated. The results showed that the % of acceleration in healing days were of 15.09, 22.93, 23.21 and 20.37 in 442nm, 514nm, 632nm, 786nm and 830nm at the incident dose of 20 J/cm 2 , respectively. The results also suggested that He Ne laser with 632nm was the most effective in promoting wound healing in all wavelength used in this study. The inhibitory effect of low power Argon laser showed the zero bioactivation at the incident dose of 80 J/cm 2 and the deceleration in healing days was –8.65% at the incident dose of 130 J/cm 2.
Computerized Morphometric Assessment of the Effect of Low Level Laser Therapy on Bone Repair: an Experimental Animal Study
Silva Júnior AN, Pinheiro AL, Oliveira MG, Weismann R, Ramalho LM, Nicolau RA. J Clin Laser Med Surg. 2002; 20: 83-87
The aim of this study was to evaluate morphometrically the amount of newly formed bone after GaAlAs laser irradiation of surgical wounds created in the femur of rats. Low-level laser therapy (LLLT) has been used in several medical specialties because of its biomodulatory effects on different biological tissues. However, LLLT is still controversial because of contradictory reports. This is a direct result of the different methodologies used in these works. In this study, 40 Wistar rats were divided into four groups of 10 animals each: group A (12 sessions, 4.8 J/cm2 per session, observation time of 28 days); group C (three sessions, 4.8 J/cm2 per session, observation time of 7 days). Groups B and D acted as nonirradiated controls. The specimens were routinely processed to wax and cut at 6-microm thickness and stained with H&E. For computerized morphometry, Imagelab software was used. RESULTS: Computerized morphometry showed a significant difference between the areas of mineralized bone in groups C and D (p = 0.017). There was no difference between groups A and B (28 days; p = 0.383).
Therapeutic Low Energy Laser Improves the Mechanical Strength of Repairing Medial Collateral Ligament
Fung DT, Ng GY, Leung MC, Tay DK. Lasers Surg Med. 2002; 31:91-96.
Twenty-four rats received surgical transection to their right MCL and eight received sham operation. After surgery, 16 received a single dose of gallium aluminum arsenide laser to their transected MCL for 7.5 minutes (n = 8) or 15 minutes (n = 8) and eight served as control with placebo laser, while the sham group didn't receive any treatment. The MCLs were biomechanically tested at either 3 or 6 weeks post-operation. The normalized ultimate tensile strength (UTS) and stiffness of laser and sham groups were larger than control (P < 0.001). The UTS of laser and sham groups were comparable. Laser and sham groups had improved in stiffness from 3 to 6 weeks (P < 0.001). A single dose of low energy laser therapy improves the UTS and stiffness of repairing MCL at 3 and 6 weeks after injury.
Systemic Effects of Low-Intensity Laser Irradiation on Skin Microcirculation in Patients with Diabetic Microangiopathy
Andreas Schindla, Georg Heinzeb, Martin Schindlc, Heidemarie Pernerstorfer-Schönd and Liesbeth Schindlca Department of Dermatology, Division of Special and Environmental Dermatology, University of Vienna Medical School, Vienna, Austria
b Department of Medical Computer Sciences, University of Vienna Medical School, Vienna, Austria
c Institute for Lasermedicine, Klosterneuburg/Vienna, Austria
d Department of Dermatology, Division of Immunodermatology and Infectious Dermatoses, University of Vienna Medical School, Vienna, Austria
Received 27 February 2002. Available online 25 August 2002.
Low-intensity laser irradiation has been shown to induce wound healing in conditions of reduced microcirculation, which is in part explained by systemic effects. We therefore investigated such a potential systemic effect of low-intensity laser irradiation on skin blood circulation in patients with diabetic microangiopathy. Patients with diabetic microangiopathy were randomized to receive either a single helium–neon (HeNe, 632.8 nm) low-intensity laser irradiation with a dose of 30 J/cm2 or a sham irradiation over the forefoot region in a double-blind, placebo-controlled clinical study. Skin blood circulation by means of temperature recordings over forefoot regions was detected by infrared thermography. Following a single transcutaneous low-intensity laser irradiation, a rise in skin temperature in both feet of the subjects in the laser group was noted, whereas in both feet of the subjects in the placebo group a drop in skin temperature occurred. The baseline-adjusted skin temperature 15 min after the end of the irradiation was significantly higher in the laser-treated forefeet compared to the placebo-“treated” forefeet (p < 0.0001); the baseline-adjusted difference in the temperature was 1.94 ± 0.35°C. Simultaneously, the baseline-adjusted skin temperature was significantly higher in the laser-untreated forefeet compared to the placebo-“untreated” forefeet (P < 0.0001); the baseline-adjusted difference was 1.70 ± 0.33°C. Our data show a significant increase in skin circulation due to athermic laser irradiation in patients with diabetic microangiopathy and point to the possibility of inducing systemic effects.
Effects of laser irradiation on human thrombus: Demonstration of a linear dissolution-dose relation between clot length and energy density
Garrett Lee MD, 1, 2, Richard M. Ikeda MD1, 2, Daniel Stobbe1, 2, Claire Ogata BS1, 2, Ming C. Chan MD1, 2, Daniel L. Seckinger MD1, 2, Anthony Vazquez1, 2, Jerold Theis DVM, PhD1, 2, Robert L. Reis MD1, 2 and Dean T. Mason MD1, 2
1From the Cedars Medical Center Cardiovascular Laser Research Laboratory, Miami, Florida, USA
2From the University of California, School of Medicine, Davis, California USA
Received 13 June 1983; accepted 17 June 1983. Available online 6 March 2004.
Because vascular thrombosis often accompanies arteriosclerotic disease in occluding blood vessels, the dissolution properties of laser irradiation were investigated and the energies needed to penetrate different lengths of thrombus were quantitated. Spectrophotometric studies show that the blood clot due to the presence of hemoglobin is well absorbed by argon laser energies, which emit blue-green wavelengths between 454 and 514 nm. Thus, laser energies transmitted directly from an argon-ion source produced vaporization and penetration of human thrombus in a linear dose-response fashion; the longer the thrombus, the greater the power intensity or time exposure necessary to penetrate the clot.
ROLE OF THE CIRCULATING BLOOD IN INITIATION OF THERAPEUTIC EFFECTS OF VISIBLE LIGHT
K.A.Samoilova Institute of Cytology of the Russian Academy of Sciences, St.
Petersburg, Russia
The successful use in Russia of the extracorporeal and intravascular irradiation of the
patient`s blood with the laser and non-laser light for the wound healing promotion,
immunomodulation, pain relief, etc. suggests that development of these effect in the case
of the percutaneous light application also is due to its effect on blood. Such mechanism
seems quite possible, as the visible and IR light penetrating the skin at the depth of 2-4
mm reaches a network of small vessels and can affect the blood that circulates here at a
low rate. Since during the laser treatment it is possible to irradiate only a small area of the
surface body or wound and, correspondingly, of a very small volume of the circulating
blood, it is necessary to explain how its photomodification can lead to a wide spectrum of
therapeutic effects. For this purpose, we refer to the data obtained at our Laboratory in
studying mechanisms of the rapidly developing functional changes induced by
retransfusions of a small blood volume (1.5-2.5% of its circulating pool) irradiated
extracorporally with the UV or He-Ne laser light. It has been found out that the optical
radiation induces structural-functional changes and activation of blood cells, which
results in that a small volume of blood acquires properties of an active preparation able to
modulate the state of cells of the autologous blood and other tissues. Indeed, when the
photomodified blood is added in vitro to the 10-80-fold volume of the intact autologous
blood, it "translates" to it the light-induced changes that, in spite of such a pronounced
dilution of blood, not only are not reduced but even are enhanced. As a result, the entire
volume of the circulating blood acquires properties of an active preparation. The chief
"translators" of the light effects are the irradiated mononuclear leukocytes and platelets,
and whereas development of some effects (improvement of hemorheology, activation of
all types of leucocytes, etc), is necessarily associated with the presence of irradiated cells,
the development of other effects (first of all an enhancement of the growth promoting
activity of blood) is initiated by soluble factors secreted by these cells. By now we have
accumulated an evidence for that after exposure of a small area of the body to visible
incoherent polarized light, immediate changes occur of the rheological, transport, gas
transport, growth stimulating, immunomodulating properties of the circulating blood and
that these changes develop mainly as a consequence of the effect of the transcutaneously
photomodified blood.
Experimental study of low level laser radiation effects on human blood
cells.
Siposan D, Adalbert L (Bucharest, Roumania).
Fresh blood from 40 apparently healthy individuals has been irradiated with a low level
HeNe laser, using EDTA anticoagulant. Doses ranged between 0-54 J/cm2. The authors
watched the relative variation to the received doses of hemoreological constants -
erythrocytary and leukocytary indices, as well as the variation of some erythrocytary
aggreagability indices-viscocity, BSR. Following irradiation a lowering of the
erythrocytary aggreagability (viscosity), BSR, and changes of some erythrocytary and
leukocytary indices have been observed. The effect of low-level laser radiation on the red
cell confirms the non-resonant mechanism of this bio-stimulating radiation effect by the
changes in the cell membrane, in our case the blood cells, by revitalizing the red blood
cell functional capacities and by several biochemical effects on the membrane level, that
are to be studied thoroughly in future studies. It is concluded that the physicalbiochemical
and biological effects on blood can influence the physical-chemical
parameters needed for long storage of blood products as well as the quick revitalization
of the erythrocytary membrane aggressed physically and biochemically, in order to
perform its oxophoric function in transfusion procedures.
Dynamics of lipid metabolism and peripheral blood flow rates in patients
with atherosclerosis in conjunction with renal dysfunction after the course
of combined laser therapy.
Kovalyova T V et al.
During an 8 year period patients with atherosclerosis and renal dysfunction have been
treated with intravenous laser blood irradiation (ILBI). The study has demonstrated a
decreased level of total cholesterol, LDL cholesterol and triglycerides with an
simultaneous increase of HDL cholesterol levels. No pharmaceuticals were given during
the treatment period. The authors state that ILBI results in a stable hypolipidemic
situation which prevents atherogenesis in patients with metabolic disorders, particularly
in patients with renal pathologies.
Cytological parameters of bronchoalveolar lavage in patients with chronic
obstructive bronchitis exposed to laser radiation of blood].
Terapevticheskii arkhiv. 1999; 71(11): 65-67.
Ananchenko V G, Khanin A G, Gostishcheva O V.
Clinicocytological evaluation of the efficacy of combined treatment of chronic
obstructive bronchitis (COB) in exacerbation with application of laser radiation of blood
was performed. Combined treatment with the use of He-Ne intravenous and
transcutaneous radiation of blood was given to 32 patients with COB. 27 COB patients
treated without blood irradiation served as control. In addition to conventional methods
of examination and control of the treatment effect, cytological and bacteriological tests of
BAL precipitate smears were made. Combined COB treatment with the use of laser blood
radiation has an antiinflammatory action, promotes normalization of mucociliary
transport, activation of phagocytosis and immune defense, cleansing of bronchial tree,
reduction of obstruction effective management of exacerbations. Hospitalization
decreased 3-4 days. Blood irradiation has the advantages as a noninvasive method.
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